Interphase Reactions

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Visualization of interphase chromosomes.

Using a modified Giemsa-banding technique we have observed what appear to be chromosomes during interphase in nuclei from Allium cepa root tips and Chinese hamster cells (CHO line). During telophase through G1 chromosomes progressively uncoil and decondense. During S chromosomes are comparatively decondensed, but some segments have structure similar to chromosomes in G1 and G2. During G2 the ch...

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Interphase Chromosome Flow-FISH.

A 2-day method using flow cytometry and FISH for interphase cells was developed to detect monosomy 7 cells in myelodysplastic syndrome patients. The method, Interphase Chromosome Flow-FISH (IC Flow-FISH), involves fixation of leukocytes from blood, membrane permeabilization, hybridization of cellular DNA with peptide nucleic acid probes with cells intact, and analysis by flow cytometry. Hundred...

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Sorting out interphase microtubules

Proper organization of microtubule polymers is crucial to the form and function of all eukaryotic cells. Whether growing, dividing or polarizing, different cell types—be they neurons, plant cells or fungal cells—organize specialized microtubule patterns appropriate for their needs. Is it possible to identify the set of factors sufficient to organize microtubules in a specific cell, and to under...

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Correlative Interphase Cytogenetic Analysis

Background: A patient with multiple myeloma developed myelodysplastic syndrome (MDS). Chromosomal analysis performed after the development of MDS revealed monosomy of chromosome 9 in all the meta-phases. We wished to identify the extent of the clone with the chromosomal abnormality originating from MDS clone. Methods: A correlative interphase study by fluorescence in situ hybridization (FISH) w...

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Entropic organization of interphase chromosomes

Chromosomes are not distributed randomly in nuclei. Appropriate positioning can activate (or repress) genes by bringing them closer to active (or inactive) compartments like euchromatin (or heterochromatin), and this is usually assumed to be driven by specific local forces (e.g., involving H bonds between nucleosomes or between nucleosomes and the lamina). Using Monte Carlo simulations, we demo...

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ژورنال

عنوان ژورنال: Journal of Japan Oil Chemists' Society

سال: 1974

ISSN: 1884-2003

DOI: 10.5650/jos1956.23.593